The issues of terrorism, protection against crime, anti-social behaviour, and sociopathological phenomena are current topics in today’s world. At present, there is no effective assessment in the Czech Republic of the physical security of buildings which could be the target of the threats. Within the security research of the Czech Republic, research was carried out whose main objective was to assess the existing level of physical security of public universities, with the subsequent determination of the minimum level of physical security of these buildings using new processes, practices, and technologies. In the Czech Republic, such research has not yet been realized. The main objective of the research was to thoroughly assess the current level of physical security of buildings at a representative sample of public universities, to create a security standard ensuring the minimum level of physical security of public universities against threats of terrorism, crime, anti-social behaviour, and also sociopathological phenomena. The contribution to the field of physical security in science is a rigorous assessment of the level of physical security measures of public universities, the analysis of criminal acts, security incidents, emergencies, risk designation, and the design of security measures. The benefit for practice is the creation of a security standard to ensure the minimum level of security of public buildings by physical security measures.